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| Registros recuperados: 15 | |
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| Pichon, Delphine; Cudennec, Benoit; Huchette, Sylvain; Djediat, Chakib; Renault, Tristan; Paillard, Christine; Auzoux-bordenave, Stephanie. |
| The decline of European abalone Haliotis tuberculata populations has been associated with various pathogens including bacteria of the genus Vibrio. Following the summer mortality outbreaks reported in France between 1998 and 2000, Vibrio harveyi strains were isolated from moribund abalones, allowing in vivo and in vitro studies on the interactions between abalone H. tuberculata and V. harveyi. This work reports the development of primary cell cultures from abalone gill tissue, a target tissue for bacterial colonisation, and their use for in vitro study of host cell—V. harveyi interactions. Gill cells originated from four-day-old explant primary cultures were successfully sub-cultured in multi-well plates and maintained in vitro for up to 24 days.... |
| Tipo: Text |
Palavras-chave: Haliotis tuberculata; Vibrio harveyi; Gill cell culture; Pathogenicity; Phenoloxidase; Phagocytosis. |
| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00146/25683/23745.pdf |
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| Harney, Ewan; Dubief, Bruno; Boudry, Pierre; Basuyaux, Olivier; Schilhabel, Markus B.; Huchette, Sylvain; Paillard, Christine; Nunes, Flavia. |
| The European abalone Haliotis tuberculata is a delicacy and consequently a commercially valuable gastropod species. Aquaculture production and wild populations are subjected to multiple climate-associated stressors and anthropogenic pressures, including rising sea-surface temperatures, ocean acidification and an emerging pathogenic Vibrio infection. Transcript expression data provides a valuable resource for understanding abalone responses to variation in the biotic and abiotic environment. To generate an extensive transcriptome, we performed next-generation sequencing of RNA on larvae exposed to temperature and pH variation and on haemolymph of adults from two wild populations after experimental infection with Vibrio harveyi. We obtained more than 1.5... |
| Tipo: Text |
Palavras-chave: RNA-Seq; Mollusca; Gastropoda; Development; Vibrio harveyi; Climate change. |
| Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00318/42940/45103.pdf |
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| Schikorski, David; Renault, Tristan; Paillard, Christine; Bidault-toffin, A.; Tourbiez, Delphine; Saulnier, Denis. |
| The Gram-negative bacterium Vibrio harveyi is known to be highly pathogenic for the European abalone Haliotis tuberculata, which is a gastronomically important marine gastropod with a high commercial value. Since 1998, some particular bacterial strains are described as implicated in recurrent mortality outbreaks in French farm and field stocks of abalone. Recently, a 9.6kb plasmid named pVCR1, was shown to be harbored by one highly V. harveyi virulent ORM4 strain suggesting its involvement in virulence phenotype. Thus, we have developed in the present study two TaqMan real-time PCR assays allowing to (i) rapidly and specifically detect, by a duplex procedure and in less than 2 h, both V. harveyi and the presence of plasmid pVCR1 from unidentified bacterial... |
| Tipo: Text |
Palavras-chave: Vibrio harveyi; Haliotis tuberculata; Real-time PCR; Plasmid; Marine pathogen; Molecular diagnostic. |
| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00124/23540/21383.pdf |
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| Sánchez Ortiz,Ileana; Martín Martín,Leonardo; García Varela,Yonarky; Abad Márquez,Zoila; Rodríguez,Ramón Franco; Ramírez Núñez,Yamilka; Zamora Sánchez,Jesús; Basulto Morales,Rosa; Moreira Rubio,Alain; Arenal Cruz,Amílcar. |
| El empleo de probióticos para el control de enfermedades en la acuicultura intensiva es una alternativa al uso de antibióticos. En este trabajo se aisló la cepa bacteriana IC1 a partir de una fermentación acido láctica de col y se identificó por técnicas moleculares y convencionales como Lactobacillus sp. Se determinó la actividad antagónica in vitro de IC1 contra cepas patrones. Para la evaluación de la actividad probiótica un grupo de postlarvas de Litopenaeus vannamei se trató cada 24 horas con 1-2 x10(4) ufc/ml de IC1 y otro se mantuvo sin tratamiento. A los 25 días se cuantificaron el peso húmedo y el comportamiento de los parámetros inmunológicos (actividad de las enzimas superóxido dismutasa, fenoloxidasa y peroxidasa). El peso de los animales que... |
| Tipo: Journal article |
Palavras-chave: Probiótico; Litopenaeus vannamei; Vibrio harveyi; Lactobacillus sp.; Fermentaciones vegetales; Bacterias ácido-lácticas. |
| Ano: 2013 |
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2013000200004 |
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| Zhou,Kang; Gui,Meng; Li,Pinglan; Xing,Shaohua; Cui,Tingting; Peng,Zhaohui. |
| Vibrio harveyi is considered as a causative agent of the systemic disease, vibriosis, which occurs in many biological fields. The effects of temperatures (12.9-27.1 ºC) and water activity (NaCl% 0.6%-3.4%) on V. harveyi were investigated. The behavior and growth characteristics of V. harveyi was studied and modeled. Growth curves were fitted by using Gompertz and Baranyi models, and the Baranyi model showed a better fittness. Then, the maximum growth rates (µmax) and lag phase durations (LPD, λ) obtained from both Gompertz and Baranyi model were modeled as a combination function of temperature and water activity using the response surface and Arrhenius-Davey models for secondary model. The value of r², MSE, bias and accuracy factor suggest Baranyi model... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Vibrio harveyi; Modelling; Temperature; Water activity. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000400018 |
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| Dubief, Bruno; Nunes, Flavia; Basuyaux, Olivier; Paillard, Christine. |
| Since 1997, populations of the European abalone Haliotis tuberculata suffer mass mortalities attributed to the bacterium Vibrio harveyi. These mortalities occur at the spawning season, when the abalone immune system is depressed, and when temperatures exceed 17 °C, leading to favorable conditions for V. harveyi proliferation. In order to identify mechanisms of disease resistance, experimental successive infections were carried out on two geographically distinct populations: one that has suffered recurrent mortalities (Saint-Malo) and one that has not been impacted by the disease (Molène). Furthermore, abalone surviving these two successive bacterial challenges and uninfected abalone were used for several post-infection analyses. The Saint-Malo population... |
| Tipo: Text |
Palavras-chave: Immunity; Hemocyte; Abalone; Disease; Extracellular products; Immune priming; Vibrio harveyi; Flow cytometry; Resistance; Phagocytosis; Bacterial growth; QPCR; Gill. |
| Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00357/46800/46667.pdf |
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| Mai, T.; Tintillier, Florent; Lucasson, Aude; Moriou, C.; Bonno, E.; Petek, S.; Magre, Kevin; Al Mourabit, A.; Saulnier, Denis; Debitus, C.. |
| Sponges are a rich source for investigation of bioactive small molecules. They have been mostly investigated for the search of new pharmacological models or therapeutic agents for the treatment of human diseases. Micro-organisms can also represent a virulent pathogen for marine invertebrates such as sponges, which need to protect themselves against these microbes. Sponges' self defence mechanisms involving dialogue molecules thus represent a pertinent research track for potent anti-infective and anti-biofilm activities such as quorum sensing inhibitors (QSIs). The investigation of the QSI crude extract of Leucetta chagosensis Dendy, 1863 led to the isolation of three new alkaloids, isonaamine D, di-isonaamidine A and leucettamine D, along with the known... |
| Tipo: Text |
Palavras-chave: Biofouling; Leucetta chagosensis; Leucettamine; Naamidine; Naamine; Quorum sensing; Quorum sensing inhibitors; Sponges; Vibrio harveyi. |
| Ano: 2015 |
URL: http://archimer.ifremer.fr/doc/00275/38597/37189.pdf |
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| Garcia-bueno, Nuria; Dumay, Justine; Guerin, Thomas; Turpin, Vincent; Paillard, Christine; Stiger-pouvreau, Valerie; Pouchus, Yves-francois; Aitor Marin-atucha, Arnaldo; Decottignies, Priscilla; Fleurence, Joel. |
| The wide polarity range and highly polar compounds of two selected red seaweed, Grateloupia turuturu and Palmaria palmata were extracted using two different types of solvent, dichloromethane/methanol and methanol/water. Monthly in vitro antibacterial activities were studied using the microplate method against the marine bacteria Vibrio harveyi strain ORM4, known to infect abalone. Inhibition, slowdown and delay of Vibrio harveyi growth were investigated. Polar compounds of seaweed showed an activity against the abalone pathogen. The best activity was recorded from P. palmata collected in spring, with an inhibition of 7.9% of the bacterial growth. Preliminary 1H NMR profiles identified the differences between the extracts. |
| Tipo: Text |
Palavras-chave: Antibacterial activity; Abalone disease; Haliotis tuberculata; Red seaweed; Vibrio harveyi. |
| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00617/72875/71929.pdf |
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| Vieira,Felipe do Nascimento; Buglione Neto,Celso Carlos; Mouriño,José Luiz Pedreira; Jatobá,Adolfo; Ramirez,Cristina; Martins,Maurício Laterça; Barracco,Margherita Anna Antonia Maria; Vinatea,Luis Alejandro. |
| The objective of this work was to assess the time-related action of probiotic Lactobacillus plantarum in the bacterial microbiota of the digestive tract of Litopenaeus vannamei, and the relation of total haemocyte count and serum phenol oxidase activity of shrimp challenged with Vibrio harveyi. Shrimps were fed with a probiotic-supplemented diet, for eight days, then shifted to a commercial diet. Shrimps fed only with the commercial diet served as control. Evaluations were made on the 8th day of experiment and repeated two, four, six and eight days later. Total lactic bacteria in the digestive tract was higher until the 4th day of evaluation in the probiotic-supplemented group. Vibrio spp. counts were higher in the control at days zero and two. Until the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Litopenaeus vannamei; Vibrio harveyi; Total haemocyte count; Lactic bacteria; Phenol oxidase; Probiotic. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2008000600013 |
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| Registros recuperados: 15 | |
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